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Addgene

MXS_EF1a
(Plasmid #62421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MXS_chaining vector
  • Backbone manufacturer
    Neveu lab, Addgene plasmid #62394
  • Backbone size w/o insert (bp) 1945
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a Promoter
  • Species
    Synthetic
  • Insert Size (bp)
    1192

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer TTACCGCCTTTGAGTGAG
  • 3′ sequencing primer TTGTCTCATGAGCGGATAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MXS_EF1a was a gift from Pierre Neveu (Addgene plasmid # 62421 ; http://n2t.net/addgene:62421 ; RRID:Addgene_62421)

  • For your References section:

    MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PONE-D-15-01385 [pii] PubMed 25909630
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