pJW1517
(Plasmid
#62387)
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PurposepFA6a Osm1(M33A)-yoEGFP::LEU2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
- Total vector size (bp) 7164
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSM1-EGFP fusion
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2244
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MutationMethionine 33 mutated to Alanine
- Promoter Adh1p
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer oCW133 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW1517 was a gift from Jonathan Weissman (Addgene plasmid # 62387 ; http://n2t.net/addgene:62387 ; RRID:Addgene_62387) -
For your References section:
Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling. Williams CC, Jan CH, Weissman JS. Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522. 10.1126/science.1257522 PubMed 25378625
