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Addgene

pJW1514
(Plasmid #62384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII blunt topo
  • Backbone size w/o insert (bp) 3519
  • Total vector size (bp) 5451
  • Vector type
    yeast c-terminal tagging vector
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tev 3x FLAG
  • Species
    Synthetic
  • Tag / Fusion Protein
    • linker-tev-linker-3xflag (C terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jimena Weibezahn

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1514 was a gift from Jonathan Weissman (Addgene plasmid # 62384 ; http://n2t.net/addgene:62384 ; RRID:Addgene_62384)

  • For your References section:

    Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling. Williams CC, Jan CH, Weissman JS. Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522. 10.1126/science.1257522 PubMed 25378625
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