Actin binding domain of Ezrin
(Plasmid #62381)

• Purpose: Expresses the actin binding domain of ezrin. Amino acids 552-586.
• Depositing Lab: Guillaume Charras
• Publication: Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. doi: 10.1016/j.bpj.2013.11.4499.

Backbone

• Vector backbone: EGFP-N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Actin binding domain of ezrin
• Species: H. sapiens (human)
• Insert Size (bp): 104
• Mutation: contains aa's 552-586
• GenBank ID: NM_001111077.1
• Entrez Gene: EZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry