FERM domain of Ezrin
(Plasmid #62380)

• Purpose: Expresses the FERM domain of Ezrin (N terminal portion of the protein)
• Depositing Lab: Guillaume Charras
• Publication: Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. doi: 10.1016/j.bpj.2013.11.4499.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 7095
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ezrin aa1-309
• Alt name: FERM domain of Ezrin (N terminal portion of the protein)
• Alt name: Ezrin
• Alt name: EZR
• Species: H. sapiens (human)
• Insert Size (bp): 888
• Mutation: contains FERM domain of Ezrin (N terminal portion of the protein, aa's 1-309)
• Entrez Gene: EZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: GFP reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry