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Addgene

pJW1234
(Plasmid #62367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62367 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7209
  • Modifications to backbone
    PGK promoter and CYC1 terminator
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA
  • Alt name
    biotin ligase
  • Insert Size (bp)
    966
  • Entrez Gene
    birA (a.k.a. b3973, ECK3965, bioR, dhbB)
  • Promoter PGK

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1234 was a gift from Jonathan Weissman (Addgene plasmid # 62367 ; http://n2t.net/addgene:62367 ; RRID:Addgene_62367)

  • For your References section:

    Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling. Jan CH, Williams CC, Weissman JS. Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. 10.1126/science.1257521 PubMed 25378630
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