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Addgene

pJW1512
(Plasmid #62366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pInducer11
  • Backbone manufacturer
    Elledge lab
  • Total vector size (bp) 14367
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG-BirA-mCherry-Sec61beta tail anchor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2049
  • GenBank ID
    10952
  • Entrez Gene
    SEC61B
  • Promoter pTRE2

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1512 was a gift from Jonathan Weissman (Addgene plasmid # 62366 ; http://n2t.net/addgene:62366 ; RRID:Addgene_62366)

  • For your References section:

    Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling. Jan CH, Williams CC, Weissman JS. Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. 10.1126/science.1257521 PubMed 25378630
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