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Addgene

pJW1506
(Plasmid #62360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Total vector size (bp) 5125
  • Vector type
    yeast targeting
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPL16B
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • HA-TEV-AVI (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site spei (not destroyed)
  • 3′ cloning site spei (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13 rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1506 was a gift from Jonathan Weissman (Addgene plasmid # 62360 ; http://n2t.net/addgene:62360 ; RRID:Addgene_62360)

  • For your References section:

    Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling. Jan CH, Williams CC, Weissman JS. Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. 10.1126/science.1257521 PubMed 25378630
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