pJW1505
(Plasmid
#62359)
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PurposeRPL16a HA-TEV-AVI pop-in/pop-out plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS306
- Total vector size (bp) 6206
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Vector typeyeast tagging
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPL16A
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)840
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Tag
/ Fusion Protein
- HA-TEV-AVI (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW1505 was a gift from Jonathan Weissman (Addgene plasmid # 62359 ; http://n2t.net/addgene:62359 ; RRID:Addgene_62359) -
For your References section:
Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling. Jan CH, Williams CC, Weissman JS. Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. 10.1126/science.1257521 PubMed 25378630