pJW1504
(Plasmid
#62358)
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PurposeRPS2 HA-TEV-AVI pop-in/pop-out plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS306
- Backbone size w/o insert (bp) 4373
- Total vector size (bp) 5100
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Vector typeyeast targeting
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPS2 fragment
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)727
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GenBank ID852754
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Tag
/ Fusion Protein
- HA-TEV-AVI (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site xbai (unknown if destroyed)
- 3′ cloning site sali (unknown if destroyed)
- 5′ sequencing primer T7 or M13 forward
- 3′ sequencing primer T3 or M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW1504 was a gift from Jonathan Weissman (Addgene plasmid # 62358 ; http://n2t.net/addgene:62358 ; RRID:Addgene_62358) -
For your References section:
Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling. Jan CH, Williams CC, Weissman JS. Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521. Epub 2014 Nov 6. 10.1126/science.1257521 PubMed 25378630
