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Addgene

PLEXIN A2 (m1)
(Plasmid #62353)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plexin A2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    563
  • Mutation
    bp 2430-3064 of Genbank ref NM_008882.2
  • Entrez Gene
    Plxna2 (a.k.a. 2810428A13Rik, AA589422, AW457381, OCT, PlexA2, Plxn2, mKIAA0463)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For antisense RNA transcription, use NotI and SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLEXIN A2 (m1) was a gift from Christiana Ruhrberg (Addgene plasmid # 62353 ; http://n2t.net/addgene:62353 ; RRID:Addgene_62353)
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