p28BIOH-LIC
(Plasmid
#62352)
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Purpose(Empty Backbone) E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerStructural Genomics Consortium
- Backbone size (bp) 7373
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Vector typeBacterial Expression
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- AviTag (N terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7-Fwd
- 3′ sequencing primer T7-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession: EF442785
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p28BIOH-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 62352 ; http://n2t.net/addgene:62352 ; RRID:Addgene_62352)
