pJZC73
(Plasmid
#62341)
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PurposesgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMP177_U6 (derived from pSico)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersMarked by mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namesgRNA
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MutationTargets sv40 promoter, sequence: GAATAGCTCAGAGGCCGAGG
- Promoter U6
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer gagatccagtttggttagtaccggg (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCOM-KRAB
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Alt nameCOM RNA binding protein fused to a KRAB domain
- Promoter CMV
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Tag
/ Fusion Protein
- KRAB (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer CCTCACATTGCCAAAAGACG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJZC73 was a gift from Wendell Lim & Stanley Qi (Addgene plasmid # 62341 ; http://n2t.net/addgene:62341 ; RRID:Addgene_62341) -
For your References section:
Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. 10.1016/j.cell.2014.11.052 PubMed 25533786