pACYC-GST
(Plasmid #62329)

• Purpose: (Empty Backbone) E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
• Depositing Lab: Cheryl Arrowsmith
• Publication: Arrowsmith Lab Plasmids (unpublished)

Backbone

• Vector backbone: pACYC-LIC+
• Backbone manufacturer: Structural Genomics Consortium
• Backbone size (bp): 8362
• Modifications to backbone: Inserted an N-terminal GST tag into the MCS
• Vector type: Bacterial Expression
• Promoter: T7-lac0
• Tags / Fusion Proteins:
  • Glutathione S-transferase (GST) tag (N terminal on backbone)
  • TEV protease cleavage site (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • pACYC-GST
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry