pACYC-LIC+
(Plasmid
#62312)
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Purpose(Empty Backbone) E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC-RIL
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Backbone manufacturerStructural Genomics Consortium
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Vector typeBacterial Expression
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- thrombin protease cleavage site (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7-Fwd
- 3′ sequencing primer T7-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYC-LIC+ was a gift from Cheryl Arrowsmith (Addgene plasmid # 62312 ; http://n2t.net/addgene:62312 ; RRID:Addgene_62312)
Map uploaded by the depositor.
