pET32a-LIC
(Plasmid
#62310)
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Purpose(Empty Backbone) E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET32a
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Backbone manufacturerStructural Genomics Consortium
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Vector typeBacterial Expression
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- Trx-Tag (N terminal on backbone)
- 6xHis (N terminal on backbone)
- S-tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7-Fwd
- 3′ sequencing primer T7-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession: EF442786
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET32a-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 62310 ; http://n2t.net/addgene:62310 ; RRID:Addgene_62310)
Map uploaded by the depositor.
