pAN-OR-MalT-3NT
(Plasmid #62309)

• Purpose: synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome
• Depositing Lab: Christopher Voigt
• Publication: Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.

Backbone

• Vector backbone: unknown
• Backbone size w/o insert (bp): 5133
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: A2NT sgRNA
• Species: Synthetic
• Promoter: PPhlF

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: Malt-3NT sgRNA
• Species: Synthetic
• Promoter: PA2

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 3

• Gene/Insert name: A2NT sgRNA
• Species: Synthetic
• Promoter: pBAD

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: pBAD-F

Resource Information

• Supplemental Documents:
  • pAN-OR-MalT-3NT sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We designed sgRNA‐MalT‐3NT to target the non‐template strand of the protein coding sequence from the 110th to the 117th codon.