pDSAR-Cre-F2A-I-SceI
(Plasmid
#62302)
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PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDSAR
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Backbone manufacturerMarois lab
- Backbone size w/o insert (bp) 4407
- Total vector size (bp) 9474
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase, I-SceI nuclease
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)9474
- Promoter vasa
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDSAR-Cre-F2A-I-SceI was a gift from Eric Marois (Addgene plasmid # 62302 ; http://n2t.net/addgene:62302 ; RRID:Addgene_62302) -
For your References section:
Tools for Anopheles gambiae Transgenesis. Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E. G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808. 10.1534/g3.115.016808 PubMed 25869647
