pENTR L1-vas2-Cre-L4
(Plasmid
#62301)
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PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR P1-P4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 7883
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase under control of vasa promoter and terminator
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)4539
- Promoter vasa
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on insert)
- HA (N terminal on insert)
- SV40 NLS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bytranposase from M Fraser laboratory vasa regulatory regions from A Crisanti laboratory
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR L1-vas2-Cre-L4 was a gift from Eric Marois (Addgene plasmid # 62301 ; http://n2t.net/addgene:62301 ; RRID:Addgene_62301) -
For your References section:
Tools for Anopheles gambiae Transgenesis. Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E. G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808. 10.1534/g3.115.016808 PubMed 25869647