pENTR R4-vas2-integrase-R3
(Plasmid #62299)

• Purpose: phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter
• Depositing Lab: Eric Marois
• Publication: Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808.

Backbone

• Vector backbone: pDONR P4r-P3r
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 7990
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phageC31 integrase under control of vasa promoter and terminator
• Species: Streptomyces phage C31
• Insert Size (bp): 5287
• Promoter: vasa
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• A portion of this plasmid was derived from a plasmid made by: integrase from K Basler laboratory vasa regulatory regions from A Crisanti laboratory
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry