pattB-RfB2
(Plasmid
#62296)
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Purpose(Empty Backbone) docking site transgenesis plasmid for Gateway Cloning and insect transgenesis
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
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Backbone manufacturerStratagene
- Backbone size (bp) 4884
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Modifications to backboneattB site for docking-site based transgenesis, Gateway cassette for Gateway cloning
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pattB-RfB2 was a gift from Eric Marois (Addgene plasmid # 62296 ; http://n2t.net/addgene:62296 ; RRID:Addgene_62296) -
For your References section:
Tools for Anopheles gambiae Transgenesis. Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E. G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808. 10.1534/g3.115.016808 PubMed 25869647
