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Addgene

pDSAP
(Plasmid #62293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62293 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR P4r-P3r
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5456
  • Modifications to backbone
    LacZ cassette for blue-white selection flanked by BsaI sites for GoldenGate cloning of inserts. SV40 terminator. OpIE2-pac puromycin resistance marker to select transgenic insects.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • 5′ sequencing primer M13F (after cloning! present twice before cloning)
  • 3′ sequencing primer M13R (after cloning! present twice before cloning)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDSAP was a gift from Eric Marois (Addgene plasmid # 62293 ; http://n2t.net/addgene:62293 ; RRID:Addgene_62293)

  • For your References section:

    Tools for Anopheles gambiae Transgenesis. Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E. G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808. 10.1534/g3.115.016808 PubMed 25869647
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