pcDNA3.1-CHRNA7
(Plasmid #62276)

• Purpose: mammalian expression of human Alpha7 (CHRNA7)
• Depositing Labs: Sherry Leonard, Henry Lester
• Publication: Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 10.1074/jbc.M114.582858. Epub 2014 Jul 23.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHRNA7
• Alt name: hAlpha7
• Alt name: Human Alpha7 neuronal nicotinic acetylcholine receptor gene
• Species: H. sapiens (human)
• Insert Size (bp): 1638
• GenBank ID: NM_000746.4
• Entrez Gene: CHRNA7 (a.k.a. CHRNA7-2, NACHRA7)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CGCTGCAGCTCCGGGACTCAACATG
• 3′ sequencing primer: TGCCCATCTGTGAGTTTTCCACATG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Sherry Leonard Department of Psychiatry, University of Colorado, Denver, CO 80045
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA