RHD_exon4_TALEN_L
(Plasmid
#62241)
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Purposeexpression of TALEN targeting human RHD exon4
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 3739
- Total vector size (bp) 6763
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Modifications to backbonewith a cmv promoter but without neo selection cassette
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman RHD_exon4_TALEN_Left
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Insert Size (bp)3024
- Promoter CMV
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Tag
/ Fusion Protein
- HA tag, NLS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe plasmid is purchased form Toolgen (Seoul, Korea).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RHD_exon4_TALEN_L was a gift from Hyongbum Kim (Addgene plasmid # 62241 ; http://n2t.net/addgene:62241 ; RRID:Addgene_62241) -
For your References section:
Rh D blood group conversion using transcription activator-like effector nucleases. Kim YH, Kim HO, Baek EJ, Kurita R, Cha HJ, Nakamura Y, Kim H. Nat Commun. 2015 Jun 16;6:7451. doi: 10.1038/ncomms8451. 10.1038/ncomms8451 PubMed 26078220
Map uploaded by the depositor.
