CyTERM-mGFP
(Plasmid #62237)

• Purpose: ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
• Depositing Lab: Erik Snapp
• Publication: Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20.

Backbone

• Vector backbone: clontech N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4900
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CyTERM-mGFP
• Species: Synthetic; rabbit
• Insert Size (bp): 936
• Mutation: signal anchor of rabbit cytochrome p450 and monomerized EGFP
• Promoter: CMV
• Tag / Fusion Protein:
  • mGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: tggtttagtgaaccgt
• 3′ sequencing primer: GTTCAGGGGGAGGTGTG

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used for assessing the likelihood of a fluorescent protein to oligomerize in cells. This is the monomeric control protein that does NOT form Organized Smooth Endoplasmic Reticulum (OSER) structures.