psecBFP2
(Plasmid
#62230)
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Purposeencodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneclontech N1
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4700
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesecBFP2
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SpeciesSynthetic
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Insert Size (bp)705
- Promoter cmv
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Tag
/ Fusion Protein
- secbfp2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer tggtttagtgaaccgt
- 3′ sequencing primer GTTCAGGGGGAGGTGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psecBFP2 was a gift from Erik Snapp (Addgene plasmid # 62230 ; http://n2t.net/addgene:62230 ; RRID:Addgene_62230) -
For your References section:
Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway. Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp EL. Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19. 10.1016/j.bbrc.2012.12.028 PubMed 23257162