pTargetF Citations (60)
Originally described in: Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. PubMed Journal
Articles Citing pTargetF
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One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces.
Huang H, Zheng G, Jiang W, Hu H, Lu Y.
Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3.
PubMed
Associated Plasmids |
| Growth rate control of flagellar assembly in Escherichia coli strain RP437. Sim M, Koirala S, Picton D, Strahl H, Hoskisson PA, Rao CV, Gillespie CS, Aldridge PD. Sci Rep. 2017 Jan 24;7:41189. doi: 10.1038/srep41189. PubMed |
| Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM. Liu X, Tian J, Liu L, Zhu T, Yu X, Chu X, Yao B, Wu N, Fan Y. Sci Rep. 2017 Jul 28;7(1):6786. doi: 10.1038/s41598-017-06988-1. PubMed |
| Making point mutations in Escherichia coli BL21 genome using the CRISPR-Cas9 system. Wang X, He J, Le K. FEMS Microbiol Lett. 2018 Jul 1;365(14). pii: 4955551. doi: 10.1093/femsle/fny060. PubMed |
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH.
Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x.
PubMed
Associated Plasmids |
| CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis. Wu Y, Chen T, Liu Y, Lv X, Li J, Du G, Ledesma-Amaro R, Liu L. Metab Eng. 2018 Sep;49:232-241. doi: 10.1016/j.ymben.2018.08.012. Epub 2018 Aug 31. PubMed |
| BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Gordeeva J, Morozova N, Sierro N, Isaev A, Sinkunas T, Tsvetkova K, Matlashov M, Truncaite L, Morgan RD, Ivanov NV, Siksnys V, Zeng L, Severinov K. Nucleic Acids Res. 2019 Jan 10;47(1):253-265. doi: 10.1093/nar/gky1125. PubMed |
Two-step pathway for isoprenoid synthesis.
Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G.
Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 10.1073/pnas.1812935116.
PubMed
Associated Plasmids |
| Improving Cadmium Resistance in Escherichia coli Through Continuous Genome Evolution. Qin W, Zhao J, Yu X, Liu X, Chu X, Tian J, Wu N. Front Microbiol. 2019 Feb 20;10:278. doi: 10.3389/fmicb.2019.00278. eCollection 2019. PubMed |
| AcrAB-TolC Inhibition by Peptide-Conjugated Phosphorodiamidate Morpholino Oligomers Restores Antibiotic Activity in Vitro and in Vivo. Sturge CR, Felder-Scott CF, Pifer R, Pybus C, Jain R, Geller BL, Greenberg DE. ACS Infect Dis. 2019 Aug 9;5(8):1446-1455. doi: 10.1021/acsinfecdis.9b00123. Epub 2019 Jun 11. PubMed |
| RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli. Lee J, Lee DH, Jeon CO, Lee K. J Microbiol. 2019 Oct;57(10):910-917. doi: 10.1007/s12275-019-9354-6. Epub 2019 Sep 30. PubMed |
| Hydrogen Sulfide From Cysteine Desulfurase, Not 3-Mercaptopyruvate Sulfurtransferase, Contributes to Sustaining Cell Growth and Bioenergetics in E. coli Under Anaerobic Conditions. Wang J, Guo X, Li H, Qi H, Qian J, Yan S, Shi J, Niu W. Front Microbiol. 2019 Oct 11;10:2357. doi: 10.3389/fmicb.2019.02357. eCollection 2019. PubMed |
| Overexpression of AmpC Promotes Bacteriophage Lysis of Ampicillin-Resistant Escherichia coli. Wang S, Yin B, Yu L, Dang M, Guo Z, Yan G, Hu D, Gu J, Du C, Feng X, Han W, Adam YY, Sun C, Bosse JT, Lei L. Front Microbiol. 2020 Jan 8;10:2973. doi: 10.3389/fmicb.2019.02973. eCollection 2019. PubMed |
| Regulation of Microbial Metabolic Rates Using CRISPR Interference With Expanded PAM Sequences. Kim B, Kim HJ, Lee SJ. Front Microbiol. 2020 Feb 28;11:282. doi: 10.3389/fmicb.2020.00282. eCollection 2020. PubMed |
| Genetic editing of the virulence gene of Escherichia coli using the CRISPR system. Hou M, Sun S, Feng Q, Dong X, Zhang P, Shi B, Liu J, Shi D. PeerJ. 2020 Apr 6;8:e8881. doi: 10.7717/peerj.8881. eCollection 2020. PubMed |
| CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs. Lee HJ, Kim HJ, Lee SJ. Genome Res. 2020 May;30(5):768-775. doi: 10.1101/gr.257493.119. Epub 2020 Apr 23. PubMed |
| Real-time observation of CRISPR spacer acquisition by Cas1-Cas2 integrase. Budhathoki JB, Xiao Y, Schuler G, Hu C, Cheng A, Ding F, Ke A. Nat Struct Mol Biol. 2020 May;27(5):489-499. doi: 10.1038/s41594-020-0415-7. Epub 2020 May 4. PubMed |
| CRISPR-based curing and analysis of metabolic burden of cryptic plasmids in Escherichia coli Nissle 1917. Zainuddin HS, Bai Y, Mansell TJ. Eng Life Sci. 2019 Jun 3;19(6):478-485. doi: 10.1002/elsc.201900003. eCollection 2019 Jun. PubMed |
| To hunt or to rest: prey depletion induces a novel starvation survival strategy in bacterial predators. Sathyamoorthy R, Kushmaro Y, Rotem O, Matan O, Kadouri DE, Huppert A, Jurkevitch E. ISME J. 2021 Jan;15(1):109-123. doi: 10.1038/s41396-020-00764-2. Epub 2020 Sep 3. PubMed |
| The evolution of coexistence from competition in experimental co-cultures of Escherichia coli and Saccharomyces cerevisiae. Barber JN, Sezmis AL, Woods LC, Anderson TD, Voss JM, McDonald MJ. ISME J. 2021 Mar;15(3):746-761. doi: 10.1038/s41396-020-00810-z. Epub 2020 Oct 22. PubMed |
| Gene Knockout of Beneficial Plant-associated Bacillus spp. Using the CRISPR-Cas9 Double Plasmid System. Figueredo EF, Quecine MC. Methods Mol Biol. 2021;2232:173-191. doi: 10.1007/978-1-0716-1040-4_15. PubMed |
| Multi-Path Optimization for Efficient Production of 2'-Fucosyllactose in an Engineered Escherichia coli C41 (DE3) Derivative. Ni Z, Li Z, Wu J, Ge Y, Liao Y, Yuan L, Chen X, Yao J. Front Bioeng Biotechnol. 2020 Dec 3;8:611900. doi: 10.3389/fbioe.2020.611900. eCollection 2020. PubMed |
| Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells. Feng H, Guo J, Wang T, Zhang C, Xing XH. Nucleic Acids Res. 2021 Feb 22;49(3):1263-1277. doi: 10.1093/nar/gkaa1295. PubMed |
| Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression. Lee M, Ryu M, Joo M, Seo YJ, Lee J, Kim HM, Shin E, Yeom JH, Kim YH, Bae J, Lee K. PLoS Pathog. 2021 Feb 1;17(2):e1009263. doi: 10.1371/journal.ppat.1009263. eCollection 2021 Feb. PubMed |
Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli.
Okano K, Sato Y, Hizume T, Honda K.
J Biosci Bioeng. 2021 Aug;132(2):120-124. doi: 10.1016/j.jbiosc.2021.04.009. Epub 2021 May 19.
PubMed
Associated Plasmids |
| Meningitic Escherichia coli alpha-hemolysin aggravates blood-brain barrier disruption via targeting TGFbeta1-triggered hedgehog signaling. Fu J, Li L, Huo D, Yang R, Yang B, Xu B, Yang X, Dai M, Tan C, Chen H, Wang X. Mol Brain. 2021 Jul 19;14(1):116. doi: 10.1186/s13041-021-00826-2. PubMed |
| Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation. Kolata P, Efremov RG. Elife. 2021 Jul 26;10. pii: 68710. doi: 10.7554/eLife.68710. PubMed |
| Insight into the AcrAB-TolC Complex Assembly Process Learned from Competition Studies. Rajapaksha P, Ojo I, Yang L, Pandeya A, Abeywansha T, Wei Y. Antibiotics (Basel). 2021 Jul 8;10(7). pii: antibiotics10070830. doi: 10.3390/antibiotics10070830. PubMed |
| Engineering Escherichia coli for highly efficient production of lacto-N-triose II from N-acetylglucosamine, the monomer of chitin. Hu D, Wu H, Zhu Y, Zhang W, Mu W. Biotechnol Biofuels. 2021 Oct 8;14(1):198. doi: 10.1186/s13068-021-02050-5. PubMed |
| Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321. big up tri, openA and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase. Yi H, Zhang J, Ke F, Guo X, Yang J, Xie P, Liu L, Wang Q, Gao X. Front Microbiol. 2021 Sep 29;12:744284. doi: 10.3389/fmicb.2021.744284. eCollection 2021. PubMed |
| An orthogonal metabolic framework for one-carbon utilization. Chou A, Lee SH, Zhu F, Clomburg JM, Gonzalez R. Nat Metab. 2021 Oct;3(10):1385-1399. doi: 10.1038/s42255-021-00453-0. Epub 2021 Oct 21. PubMed |
| New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen. Liao X, Xing XH, Zhang C. Methods Mol Biol. 2022;2377:123-141. doi: 10.1007/978-1-0716-1720-5_7. PubMed |
| Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli. Matson MM, Cepeda MM, Zhang A, Case AE, Kavvas ES, Wang X, Carroll AL, Tagkopoulos I, Atsumi S. Metab Eng. 2022 Jan;69:50-58. doi: 10.1016/j.ymben.2021.11.002. Epub 2021 Nov 8. PubMed |
| Metabolic engineering strategies of de novo pathway for enhancing 2'-fucosyllactose synthesis in Escherichia coli. Li M, Li C, Hu M, Zhang T. Microb Biotechnol. 2022 May;15(5):1561-1573. doi: 10.1111/1751-7915.13977. Epub 2021 Nov 29. PubMed |
| Engineering for an HPV 9-valent vaccine candidate using genomic constitutive over-expression and low lipopolysaccharide levels in Escherichia coli cells. Wang K, Zhou L, Chen T, Li Q, Li J, Liu L, Li Y, Sun J, Li T, Wang Y, Kong Z, Zheng Q, Zhang J, Yu H, Gu Y, Xia N, Li S. Microb Cell Fact. 2021 Dec 20;20(1):227. doi: 10.1186/s12934-021-01719-8. PubMed |
| Development of a TNF-alpha-mediated Trojan Horse for bacteria-based cancer therapy. Hu CW, Chang YC, Liu CH, Yu YA, Mou KY. Mol Ther. 2022 Jul 6;30(7):2522-2536. doi: 10.1016/j.ymthe.2022.04.008. Epub 2022 Apr 18. PubMed |
| Engineering a suite of E. coli strains for enhanced expression of bacterial polysaccharides and glycoconjugate vaccines. Kay EJ, Mauri M, Willcocks SJ, Scott TA, Cuccui J, Wren BW. Microb Cell Fact. 2022 Apr 21;21(1):66. doi: 10.1186/s12934-022-01792-7. PubMed |
| Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells. Bayer T, Pfaff L, Branson Y, Becker A, Wu S, Bornscheuer UT, Wei R. iScience. 2022 Apr 29;25(5):104326. doi: 10.1016/j.isci.2022.104326. eCollection 2022 May 20. PubMed |
| Independent Component Analysis Identifies the Modulons Expanding the Transcriptional Regulatory Networks of Enterohemorrhagic Escherichia coli. Im H, Lee JH, Choi SH. Front Microbiol. 2022 Jun 24;13:953404. doi: 10.3389/fmicb.2022.953404. eCollection 2022. PubMed |
| Multi-level metabolic engineering of Escherichia coli for high-titer biosynthesis of 2'-fucosyllactose and 3-fucosyllactose. Li M, Li C, Luo Y, Hu M, Liu Z, Zhang T. Microb Biotechnol. 2022 Dec;15(12):2970-2981. doi: 10.1111/1751-7915.14152. Epub 2022 Sep 22. PubMed |
| The key role of indole-3-acetic acid biosynthesis by Bacillus thuringiensis RZ2MS9 in promoting maize growth revealed by the ipdC gene knockout mediated by the CRISPR-Cas9 system. Figueredo EF, Cruz TAD, Almeida JR, Batista BD, Marcon J, Andrade PAM, Hayashibara CAA, Rosa MS, Azevedo JL, Quecine MC. Microbiol Res. 2023 Jan;266:127218. doi: 10.1016/j.micres.2022.127218. Epub 2022 Sep 30. PubMed |
| Module-Guided Metabolic Rewiring for Fucosyllactose Biosynthesis in Engineered Escherichia coli with Lactose De Novo Pathway. Li M, Luo Y, Hu M, Li C, Liu Z, Zhang T. J Agric Food Chem. 2022 Nov 23;70(46):14761-14770. doi: 10.1021/acs.jafc.2c05909. Epub 2022 Nov 14. PubMed |
| High-throughput retrieval of target sequences from complex clone libraries using CRISPRi. Burian J, Libis VK, Hernandez YA, Guerrero-Porras L, Ternei MA, Brady SF. Nat Biotechnol. 2022 Nov 21. doi: 10.1038/s41587-022-01531-8. PubMed |
| Genome-scale top-down strategy to generate viable genome-reduced phages. Yuan S, Shi J, Jiang J, Ma Y. Nucleic Acids Res. 2022 Dec 9;50(22):13183-13197. doi: 10.1093/nar/gkac1168. PubMed |
| Screening of Anti-Adhesion Agents for Pathogenic Escherichia coli O157:H7 by Targeting the GrlA Activator. Hong SY, Kim BS. J Microbiol Biotechnol. 2023 Mar 28;33(3):329-338. doi: 10.4014/jmb.2207.07065. Epub 2023 Jan 28. PubMed |
| Mesenteric Adipose Tissue-Derived Klebsiella variicola Disrupts Intestinal Barrier and Promotes Colitis by Type VI Secretion System. Gong J, Yu J, Yin S, Ke J, Wu J, Liu C, Luo Z, Cheng WM, Xie Y, Chen Y, He Z, Lan P. Adv Sci (Weinh). 2023 Apr;10(12):e2205272. doi: 10.1002/advs.202205272. Epub 2023 Feb 19. PubMed |
| Characterization and Application of the Sugar Transporter Zmo0293 from Zymomonas mobilis. Zhang K, Zhang W, Qin M, Li Y, Wang H. Int J Mol Sci. 2023 Mar 20;24(6):5888. doi: 10.3390/ijms24065888. PubMed |
| Spatial transcriptome uncovers rich coordination of metabolism in E. coli K12 biofilm. Wang T, Shen P, He Y, Zhang Y, Liu J. Nat Chem Biol. 2023 Aug;19(8):940-950. doi: 10.1038/s41589-023-01282-w. Epub 2023 Apr 13. PubMed |
| Preparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli. Liu Y, Pan C, Wang K, Guo Y, Sun Y, Li X, Sun P, Wu J, Wang H, Zhu L. Microb Cell Fact. 2023 May 6;22(1):95. doi: 10.1186/s12934-023-02099-x. PubMed |
| Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on host in a gut microphysiological system. Hayes JA, Lunger AW, Sharma AS, Fernez MT, Koppes AN, Koppes R, Woolston BM. bioRxiv. 2023 May 16:2023.05.16.538950. doi: 10.1101/2023.05.16.538950. Preprint. PubMed |
| AraC-Based Biosensor for the Detection of Isoprene in E. coli. Bhat S, Banerjee A, Alagesan S. ACS Omega. 2023 Jul 18;8(30):26806-26815. doi: 10.1021/acsomega.3c01164. eCollection 2023 Aug 1. PubMed |
| Engineered phage with cell-penetrating peptides for intracellular bacterial infections. Zhao M, Tan X, Liu Z-q, Dou L, Liu D, Pan Y-j, Ma Y-f, Yu J-l. mSystems. 2023 Oct 26;8(5):e0064623. doi: 10.1128/msystems.00646-23. Epub 2023 Aug 18. PubMed |
| Neuroprotective effects of an engineered Escherichia coli Nissle 1917 on Parkinson's disease in mice by delivering GLP-1 and modulating gut microbiota. Wu H, Wei J, Zhao X, Liu Y, Chen Z, Wei K, Lu J, Chen W, Jiang M, Li S, Chen T. Bioeng Transl Med. 2022 Jun 18;8(5):e10351. doi: 10.1002/btm2.10351. eCollection 2023 Sep. PubMed |
| Electrical-biological hybrid system for carbon efficient isobutanol production. Treece TR, Pattanayak S, Matson MM, Cepeda MM, Berben LA, Atsumi S. Metab Eng. 2023 Nov;80:142-150. doi: 10.1016/j.ymben.2023.09.007. Epub 2023 Sep 20. PubMed |
| Awakening the natural capability of psicose production in Escherichia coli. Taylor JE, Palur DSK, Zhang A, Gonzales JN, Arredondo A, Coulther TA, Lechner ABJ, Rodriguez EP, Fiehn O, Didzbalis J, Siegel JB, Atsumi S. NPJ Sci Food. 2023 Oct 14;7(1):54. doi: 10.1038/s41538-023-00231-0. PubMed |
| High-Level Production of Lacto-N-neotetraose in Escherichia coli by Stepwise Optimization of the Biosynthetic Pathway. Zhang M, Zhang K, Liu T, Wang L, Wu M, Gao S, Cai B, Zhang F, Su L, Wu J. J Agric Food Chem. 2023 Nov 1;71(43):16212-16220. doi: 10.1021/acs.jafc.3c04856. Epub 2023 Oct 18. PubMed |
| Cell-Free Protein Expression in Polymer Materials. Lee MS, Lee JA, Biondo JR, Lux JE, Raig RM, Berger PN, Bernhards CB, Kuhn DL, Gupta MK, Lux MW. ACS Synth Biol. 2024 Apr 19;13(4):1152-1164. doi: 10.1021/acssynbio.3c00628. Epub 2024 Mar 11. PubMed |
| CRISPR/Cpf1-FOKI-induced gene editing in Gluconobacter oxydans. Wang X, Li D, Qin Z, Chen J, Zhou J. Synth Syst Biotechnol. 2024 Mar 4;9(2):369-379. doi: 10.1016/j.synbio.2024.02.009. eCollection 2024 Jun. PubMed |
| Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling. Rill A, Zhao L, Bode HB. Microb Cell Fact. 2024 Apr 1;23(1):98. doi: 10.1186/s12934-024-02363-8. PubMed |
| The inactivation of tolC sensitizes Escherichia coli to perturbations in lipopolysaccharide transport. Zhu S, Alexander MK, Paiva TO, Rachwalski K, Miu A, Xu Y, Verma V, Reichelt M, Dufrene YF, Brown ED, Cox G. iScience. 2024 Mar 27;27(5):109592. doi: 10.1016/j.isci.2024.109592. eCollection 2024 May 17. PubMed |
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