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Addgene

Teto Ngn1
(Plasmid #62222)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62222 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFU Teto
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neurog1
  • Alt name
    Ngn1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    800
  • Entrez Gene
    Neurog1 (a.k.a. AKA, Math4C, Neurod3, bHLHa6, ngn1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sbfi (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Teto F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Teto Ngn1 was a gift from Kristin Baldwin (Addgene plasmid # 62222 ; http://n2t.net/addgene:62222 ; RRID:Addgene_62222)

  • For your References section:

    Selective conversion of fibroblasts into peripheral sensory neurons. Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams D, Sanna PP, Baldwin KK. Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 Nov 24. 10.1038/nn.3887 PubMed 25420069
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