Teto BRN3A
(Plasmid #62221)

• Purpose: human BRN3A cDNA
• Depositing Lab: Kristin Baldwin
• Publication: Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 Nov 24.

Backbone

• Vector backbone: pFU Teto
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRN3A
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: deleted a.a. 142-181
• GenBank ID: 5457
• Entrez Gene: POU4F1 (a.k.a. ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry