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Addgene

pCGS652
(Plasmid #62220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSK
  • Modifications to backbone
    Truncation of the pC-GoldyTALEN backbone (addgene #38143) to create a shorter C-terminal region (18 AA).
  • Vector type
    Mammalian Expression, TALEN
  • Promoter miniCaggs
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on backbone)
    • AcV5 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGS652 was a gift from Daniel Voytas (Addgene plasmid # 62220 ; http://n2t.net/addgene:62220 ; RRID:Addgene_62220)

  • For your References section:

    Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration. Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, Danuser G. Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 10.1016/j.cels.2016.08.007. 10.1016/j.cels.2016.08.007 PubMed 27667364
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