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Addgene

pT1QciMP
(Plasmid #62216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Backbone size (bp) 2644
  • Vector type
    Zebrafish transgenesis
  • Promoter QUAS-E1b

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    QUAS promoter received from Halpern lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zebrafish transgenesis vector: tol1 arms, QUAS-E1b promoter, ubiquitin C intron, multiple cloning site, ocean pout antifreeze protein terminator.
Co-inject with tol1 (not tol2) transposase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT1QciMP was a gift from Harold Burgess (Addgene plasmid # 62216 ; http://n2t.net/addgene:62216 ; RRID:Addgene_62216)

  • For your References section:

    Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA. Nucleic Acids Res. 2015 Jan 27. pii: gkv035. 10.1093/nar/gkv035 PubMed 25628360
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