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PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62213 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 4725
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Vector typemRNA synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameepNTR
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Alt namezebrafish optimized nfsB.zf1 with T41L N71S F124W
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SpeciesSynthetic
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Insert Size (bp)654
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (dam) (not destroyed)
- 5′ sequencing primer sp6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-epNTR was a gift from Harold Burgess (Addgene plasmid # 62213 ; http://n2t.net/addgene:62213 ; RRID:Addgene_62213) -
For your References section:
Direct activation of the Mauthner cell by electric field pulses drives ultrarapid escape responses. Tabor KM, Bergeron SA, Horstick EJ, Jordan DC, Aho V, Porkka-Heiskanen T, Haspel G, Burgess HA. J Neurophysiol. 2014 Aug 15;112(4):834-44. doi: 10.1152/jn.00228.2014. Epub 2014 May 21. 10.1152/jn.00228.2014 PubMed 24848468