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Addgene

pnos-Fok1:dCas9-nos
(Plasmid #62210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pnos
  • Backbone manufacturer
    Johannes Bischof and Konrad Basler
  • Backbone size w/o insert (bp) 9200
  • Total vector size (bp) 13981
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fok1-dCas9
  • Insert Size (bp)
    4770
  • Promoter nanos

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGTATCGCGAATACTTCAGTTG
  • 3′ sequencing primer CTGGTTAACCCAGCTTTGATCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit crisprflydesign.org for more information.

Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnos-Fok1:dCas9-nos was a gift from Simon Bullock (Addgene plasmid # 62210 ; http://n2t.net/addgene:62210 ; RRID:Addgene_62210)
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