pBUN421
(Plasmid #62204)

• Purpose: CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
• Depositing Lab: Qi-Jun Chen
• Publication: Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Backbone

• Vector backbone: pGreen-like
• Backbone size w/o insert (bp): 3200
• Vector type: Plant Expression ; plant binary vector
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: gRNA scaffold
• Species: Synthetic
• Promoter: TaU3

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Spect-F

Gene/Insert 2

• Gene/Insert name: zCas9
• Species: S. pyogenes
• Mutation: maize codon optimized
• Promoter: Ubi
• Tags / Fusion Proteins:
  • 3x FLAG (N terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry