pMuLE ENTR SV40 PuroR L5-L2
(Plasmid
#62165)
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PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeMammalian Expression ; MuLE Gateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePuromycin
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SpeciesSynthetic
- Promoter SV40
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMuLE ENTR SV40 PuroR L5-L2 was a gift from Ian Frew (Addgene plasmid # 62165 ; http://n2t.net/addgene:62165 ; RRID:Addgene_62165) -
For your References section:
A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. 10.1172/JCI79743 PubMed 25751063