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Addgene

pMuLE ENTR U6 n.s shRNA L5-L2
(Plasmid #62139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Mammalian Expression, RNAi ; MuLE Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    non-silencing (scrambled) shRNA
  • Species
    Synthetic
  • Promoter U6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the n.s. shRNA contains an additional "C" nucleotide compared to the depositors reference sequence. The sequence between AgeI and EcoRI is 5' -ccggtcCtaaggttaagtcgccctcgctcgagcgagggcgacttaaccttaggtttttg - 3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE ENTR U6 n.s shRNA L5-L2 was a gift from Ian Frew (Addgene plasmid # 62139 ; http://n2t.net/addgene:62139 ; RRID:Addgene_62139)

  • For your References section:

    A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. 10.1172/JCI79743 PubMed 25751063
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