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Addgene

pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2
(Plasmid #62130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Mammalian Expression, CRISPR ; MuLE Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2 was a gift from Ian Frew (Addgene plasmid # 62130 ; http://n2t.net/addgene:62130 ; RRID:Addgene_62130)

  • For your References section:

    A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. 10.1172/JCI79743 PubMed 25751063
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