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pET28-p53 (1-73) (K24N)
(Plasmid #62079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28A
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Alt name
    p53 (1-73) (K24N)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    219
  • Mutation
    contains TAD residues 1-73; K24N
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes a thrombin cleavage site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-p53 (1-73) (K24N) was a gift from Gary Daughdrill (Addgene plasmid # 62079 ; http://n2t.net/addgene:62079 ; RRID:Addgene_62079)

  • For your References section:

    Impact of the K24N mutation on the transactivation domain of p53 and its binding to murine double-minute clone 2. Zhan YA, Wu H, Powell AT, Daughdrill GW, Ytreberg FM. Proteins. 2013 Oct;81(10):1738-47. doi: 10.1002/prot.24310. Epub 2013 Jul 22. 10.1002/prot.24310 PubMed 23609977
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