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pET28A-p53 (1-73) (P to A)
(Plasmid #62069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28A
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Alt name
    p53 (1-73) (PtoA)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    219
  • Mutation
    Contains TAD residues 1-73; all Prolines changed to Alanines
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes a thrombin cleavage site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28A-p53 (1-73) (P to A) was a gift from Gary Daughdrill (Addgene plasmid # 62069 ; http://n2t.net/addgene:62069 ; RRID:Addgene_62069)

  • For your References section:

    Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT, Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW. Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. Epub 2014 Nov 2. 10.1038/nchembio.1668 PubMed 25362358
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