pGEX6P2-MDMX (23-111)
(Plasmid
#62064)
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PurposeExpression of human MDM4 (residues 23-111) in e. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePGEX-6P-2
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Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4985
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMDM4
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Alt nameMDM4 (residues 23-111)
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Alt nameMDM4 proto-oncogene, E3 ubiquitin
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SpeciesH. sapiens (human)
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Insert Size (bp)269
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Mutationcontains residues 23-111
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Entrez GeneMDM4 (a.k.a. BMFS6, HDMX, MDMX, MRP1)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes a prescission cleavage site
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P2-MDMX (23-111) was a gift from Gary Daughdrill (Addgene plasmid # 62064 ; http://n2t.net/addgene:62064 ; RRID:Addgene_62064) -
For your References section:
Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT, Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW. Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. Epub 2014 Nov 2. 10.1038/nchembio.1668 PubMed 25362358