pGEX6P2-MDM2 (17-125)
(Plasmid #62063)

• Purpose: Expression of human MDM2 (17-125) in e. coli
• Depositing Lab: Gary Daughdrill
• Publication: Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. Epub 2014 Nov 2.

Backbone

• Vector backbone: PGEX-6P-2
• Backbone manufacturer: GE Healthcare Life Sciences
• Backbone size w/o insert (bp): 4985
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDM2
• Alt name: MDM2 (residues 17-125)
• Alt name: MDM2 proto-oncogene, E3 ubiquitin protein ligase
• Alt name: hdm2
• Species: H. sapiens (human)
• Insert Size (bp): 318
• Mutation: contains residues 17-125
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Includes prescission cleavage site
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Prescission cleavage.