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pET28-p53 (Mouse 1-87)
(Plasmid #62061)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62061 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28A
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Alt name
    Mouse p53 residues 1-87
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    261
  • Mutation
    contains TAD residues 1-87
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes a thrombin cleavage site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-p53 (Mouse 1-87) was a gift from Gary Daughdrill (Addgene plasmid # 62061 ; http://n2t.net/addgene:62061 ; RRID:Addgene_62061)

  • For your References section:

    Disorder predictors also predict backbone dynamics for a family of disordered proteins. Daughdrill GW, Borcherds WM, Wu H. PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub 2011 Dec 15. 10.1371/journal.pone.0029207 PubMed 22195023
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