NET29A.Mm pEGFP-N2 (1326)
(Plasmid
#62045)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNET29A
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Alt nameTmem120a
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SpeciesM. musculus (mouse)
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Entrez GeneTmem120a (a.k.a. 2010310D06Rik, Tmpit)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
assembled from 2 pieces
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NET29A.Mm pEGFP-N2 (1326) was a gift from Eric Schirmer (Addgene plasmid # 62045 ; http://n2t.net/addgene:62045 ; RRID:Addgene_62045)
