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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMARCHV
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Alt nameNKP66
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SpeciesH. sapiens (human)
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Entrez GeneMARCHF5 (a.k.a. MARCH-V, MARCH5, MITOL, RNF153)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NKP66 pEGFP-N2 (1176) was a gift from Eric Schirmer (Addgene plasmid # 62039 ; http://n2t.net/addgene:62039 ; RRID:Addgene_62039) -
For your References section:
A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal. Korfali N, Srsen V, Waterfall M, Batrakou DG, Pekovic V, Hutchison CJ, Schirmer EC. PLoS One. 2011 Apr 14;6(4):e18762. doi: 10.1371/journal.pone.0018762. 10.1371/journal.pone.0018762 PubMed 21533191
