NET5L pEGFP-N2 (1155)
(Plasmid
#62035)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNET5L
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Alt nameSAMP1, Imo2
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SpeciesH. sapiens (human)
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Mutationlong form
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Entrez GeneTMEM201 (a.k.a. Ima1, NET5, SAMP1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Long isoform of NET5 with G386E relative to closest reference sequence (XP_008973893.1).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NET5L pEGFP-N2 (1155) was a gift from Eric Schirmer (Addgene plasmid # 62035 ; http://n2t.net/addgene:62035 ; RRID:Addgene_62035) -
For your References section:
Several novel nuclear envelope transmembrane proteins identified in skeletal muscle have cytoskeletal associations. Wilkie GS, Korfali N, Swanson SK, Malik P, Srsen V, Batrakou DG, de las Heras J, Zuleger N, Kerr AR, Florens L, Schirmer EC. Mol Cell Proteomics. 2011 Jan;10(1):M110.003129. doi: 10.1074/mcp.M110.003129. Epub 2010 Sep 27. 10.1074/mcp.M110.003129 PubMed 20876400