NET5s pEGFP-N2 (1154)
(Plasmid
#62034)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNET5s
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Alt nameSAMP1, Imo1
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SpeciesH. sapiens (human)
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Mutationshort form
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Entrez GeneTMEM201 (a.k.a. Ima1, NET5, SAMP1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Short isoform of NET5 containing G411E relative to closest reference sequence XP_006710480.1.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NET5s pEGFP-N2 (1154) was a gift from Eric Schirmer (Addgene plasmid # 62034 ; http://n2t.net/addgene:62034 ; RRID:Addgene_62034) -
For your References section:
Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope. Malik P, Korfali N, Srsen V, Lazou V, Batrakou DG, Zuleger N, Kavanagh DM, Wilkie GS, Goldberg MW, Schirmer EC. Cell Mol Life Sci. 2010 Apr;67(8):1353-69. doi: 10.1007/s00018-010-0257-2. Epub 2010 Jan 21. 10.1007/s00018-010-0257-2 PubMed 20091084