RHBDD1 pEGFP-N2 (1078)
(Plasmid
#62028)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRHBDD1
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Alt nameNET82
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SpeciesH. sapiens (human)
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Entrez GeneRHBDD1 (a.k.a. RHBDL4, RRP4)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RHBDD1 pEGFP-N2 (1078) was a gift from Eric Schirmer (Addgene plasmid # 62028 ; http://n2t.net/addgene:62028 ; RRID:Addgene_62028) -
For your References section:
Several novel nuclear envelope transmembrane proteins identified in skeletal muscle have cytoskeletal associations. Wilkie GS, Korfali N, Swanson SK, Malik P, Srsen V, Batrakou DG, de las Heras J, Zuleger N, Kerr AR, Florens L, Schirmer EC. Mol Cell Proteomics. 2011 Jan;10(1):M110.003129. doi: 10.1074/mcp.M110.003129. Epub 2010 Sep 27. 10.1074/mcp.M110.003129 PubMed 20876400
