NKP33 GFP (1032)
(Plasmid
#62022)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGPF-N2
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Backbone manufacturerunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC9orf46
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Alt nameNKP33
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SpeciesH. sapiens (human)
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Entrez GenePLGRKT (a.k.a. AD025, C9orf46, MDS030, PLG-RKT, Plg-R(KT))
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NKP33 GFP (1032) was a gift from Eric Schirmer (Addgene plasmid # 62022 ; http://n2t.net/addgene:62022 ; RRID:Addgene_62022) -
For your References section:
The nuclear envelope proteome differs notably between tissues. Korfali N, Wilkie GS, Swanson SK, Srsen V, de Las Heras J, Batrakou DG, Malik P, Zuleger N, Kerr AR, Florens L, Schirmer EC. Nucleus. 2012 Nov-Dec;3(6):552-64. doi: 10.4161/nucl.22257. Epub 2012 Sep 18. 10.4161/nucl.22257 PubMed 22990521