NKP38 GFP (1009)
(Plasmid #62021)

• Purpose: mammalian expression of nuclear envelope transmembrane protein
• Depositing Lab: Eric Schirmer
• Publication: Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi: 10.4161/nucl.22257. Epub 2012 Sep 18.

Backbone

• Vector backbone: pEGPF-N2
• Backbone manufacturer: unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AYTL1
• Alt name: NKP38
• Species: H. sapiens (human)
• Entrez Gene: LPCAT2 (a.k.a. AGPAT11, AYTL1, LPLAT9, LysoPAFAT)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry