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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLBR
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SpeciesH. sapiens (human)
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Entrez GeneLBR (a.k.a. C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mRFP1-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LBR pEGFP-N2 (646) was a gift from Eric Schirmer (Addgene plasmid # 61996 ; http://n2t.net/addgene:61996 ; RRID:Addgene_61996) -
For your References section:
System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev AB, Schirmer EC. J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. 10.1083/jcb.201009068 PubMed 21444689